100 nm IVD‑READY Gold Nanoparticles for Biosensing

NanoBrand 100 nm IVD‑READY Gold Nanoparticles are optimized for high‑signal IVD assays that require clear, easily interpretable visual readouts. Their large core size delivers intense plasmonic color and a strong optical response, making them ideal for visually read lateral flow assays (LFAs), colorimetric IVD tests, and instrumented biosensing platforms where line clarity, contrast, and sensitivity are critical for diagnostic accuracy and user confidence.

- Application focus: IVD biosensing, rapid tests, ELISA, microfluidics, biosensors, LFAs

- Format: Aqueous colloidal suspension, ready for bioconjugation

- Grade: Research and development, suitable for transition to commercial IVD use

High monodispersity for reliable diagnostics

Core diameter: 100 nm, narrow size distribution

CoV (coefficient of variation): ≤ 5%

Polydispersity index: PdI ≤ 0.1

Tightly controlled monodispersity ensures uniform localized surface plasmon resonance (LSPR), generating a strong, consistent red signal and predictable optical response in IVD biosensing assays. This uniformity improves lot‑to‑lot reproducibility and provides more reliable limits of detection than polydisperse gold nanoparticle colloids. The narrow size distribution and clean, reactant‑free surface chemistry support robust antibody and biomolecule conjugation, enabling sensitive, reproducible detection of proteins, antibodies, and nucleic acids in clinical diagnostics and research applications. Explore more about the Benefits of monodisperse Gold Nanoparticles for LFAs.

Clean surface and reactant‑free matrix

Morphology: spherical gold nanoparticles, no other shapes. 

Matrix: reactant‑free aqueous colloidal suspension, low ionic strength.

The reactant‑free and stabilizer-free formulation minimizes interference with surface functionalization and reduces aggregation risk, helping maintain colloidal stability, signal intensity, and assay robustness over time. Find out Why should Stabilizing agents be Avoided.

Surface options for flexible conjugation

- Citrate: for passive adsorption of antibodies and proteins, ideal for rapid screening and conjugation optimization.

- PEG5000‑carboxylate (thiol‑PEG‑COOH): for covalent EDC/NHS coupling to amine‑containing biomolecules, with reduced non‑specific binding and enhanced stability in complex matrices.

This flexibility lets align your conjugation strategy with assay format, stability requirements, and IVD regulatory needs. Find out Why is Surface Chemistry a "real product" for Gold Nanoparticles.

Optimized OD for biosensing

Optical density options: OD 20 and OD 50. These concentrations are tuned for robust conjugation performance and reliable signal generation in common biosensing formats. 

IVD‑oriented quality control and CoA

Each lot includes a detailed CoA with lot‑specific TEM images, size distribution, UV‑Vis spectra, hydrodynamic diameter, PdI, zeta potential, and key QC parameters, supporting internal QC, method validation, and regulatory submissions for IVD and medical diagnostic products.

Consult a typical CoA for 100nm Gold Nano-Spheres, PEG5K-CARB, OD=50 and CoA for 100nm Gold Nano-Spheres, Citrate, OD=20.

When to choose 100 nm

The 100 nm IVD‑READY Gold Nanoparticles format is ideal for in vitro diagnostic assays that require maximum signal strength and clear line visibility at the test or capture zone. It is particularly suited for low‑abundance analytes, point‑of‑care tests for untrained users, and as a high‑signal reference during lateral flow assay (LFA) and IVD biosensor optimization and scale‑up. Choose 100 nm IVD‑READY Gold Nanoparticles when you need maximum visual contrast on test and control lines and high signal per binding event at low analyte concentrations, using this size as a high‑signal benchmark against smaller diameters. Learn more about Gold Nanoparticles in Biosensing and Personalized Medicine.

Customization and OEM Options

Custom diameters, optical densities, and PEG spacer lengths are available on request. Contact us to discuss your specific requirements.

Storage and Stability

- Citrate‑coated: store at 4–25 °C, do not freeze. Gently shake before use.

- PEG‑COOH: store at 2–8 °C, do not freeze. Gently shake before use.

Recommended storage conditions are indicated on the CoA. Under these conditions, products are typically stable for at least 12 months. For long‑term storage, check UV‑Vis spectra periodically to confirm colloidal and optical stability.

Safety and Handling

NanoBrand does not guarantee the suitability of its products for any particular application. It is the customer’s responsibility to ensure that the product is appropriate and permitted for the intended use, in accordance with applicable regulations and internal quality systems. Please consult our Terms of Service and follow institutional guidelines for the safe handling and disposal of nanomaterials.

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