80 nm ''Lateral-Flow-Easy'' Gold Nanoparticles

80 nm Gold Nanoparticles “Lateral‑Flow‑Easy” are highly monodisperse nano‑spheres specifically engineered for lateral flow assays (LFAs) and other immunochromatographic formats. Their tight size distribution and optimized surface chemistry deliver high‑contrast test and control lines with minimal background, supporting robust assay development, validation, and scale‑up for diagnostic applications.

These LFA‑grade gold nanoparticles combine excellent monodispersity with carefully tuned optical and flow properties to ensure predictable performance on nitrocellulose and other LFA membranes. Uniform size leads to consistent localized surface plasmon resonance (LSPR) and a stable, intense red color signal that is critical for visual detection, while avoiding the diffuse or inconsistent colors associated with more polydisperse colloids.

Particle and Surface Properties:

• Core diameter: 80 nm, narrow size distribution (CoV ≤ 5%, PdI ≤ 0.1) 
• Morphology: spherical gold nanoparticles, no other shapes
• Surface options: citrate‑coated formulation or PEG‑COOH (thiol‑PEG‑carboxylate)
• Matrix: reactant‑free aqueous colloidal suspension, low ionic strength
• Supplied with: Lot-specific Certificate of Analysis

Consult a typical CoA for 80nm Gold Nano-Spheres, PEG5K-CARB, OD=50 and CoA for 80nm Gold Nano-Spheres, Citrate, OD=20.

PEG‑Carboxylate Surface

PEG chains are attached via gold–thiol bonds, leaving terminal carboxyl groups available for covalent coupling. This highly stable coating offers excellent resistance to nonspecific protein adsorption, high‑salt and pH stability, and enables EDC/NHS coupling to amine‑containing proteins and biomolecules for preparation of stable, covalent conjugates.

Advantages in Lateral Flow Assays:

Consistent Optical Properties

Monodisperse 80 nm gold nano‑spheres exhibit uniform LSPR characteristics, resulting in a stable and predictable red color on LFA test and control lines. This consistency improves readability, reduces assay‑to‑assay variability, and is particularly important for commercial diagnostic tests where visual clarity and user confidence are critical.

Improved Sensitivity and Limit of Detection

The uniform size of these nanoparticles supports optimal binding efficiency to antibodies, antigens, and other biomolecular recognition elements. Enhanced per‑particle signal and strong plasmonic absorption at the test line translate into improved analytical sensitivity and lower limits of detection compared with polydisperse or non‑optimized colloidal gold.

Enhanced Reproducibility and Flow Dynamics

Highly monodisperse particles ensure consistent conjugation with recognition elements and reproducible capillary flow through nitrocellulose membranes. This minimizes batch‑to‑batch variation in conjugate performance, reduces the risk of clogging or uneven particle distribution, and supports dependable scale‑up from R&D to pilot and full‑scale manufacturing.

High Colloidal Stability

Reactant‑free dispersions with controlled surface chemistry yield high colloidal stability and reduced aggregation risk. Stable particles maintain their optical properties and mobility during storage and throughout the assay, preventing false signals, flow issues, or performance drift over time.

Tunable Size and Surface for Assay Optimization

Within the “Lateral‑Flow‑Easy” product line, gold nanoparticles can be synthesized at specific diameters and supplied with different concentrations and PEG spacer lengths. This tunability allows researchers to optimize signal strength, flow behavior, and conjugation strategy for particular analytes, membrane types, and assay formats.

Explore more about the Benefits of monodisperse gold nanoparticles for LFAs.

Manufacturing and Scale‑Up Consistency

Gold nanoparticles are manufactured under tightly controlled synthesis and quality‑control procedures. This high level of reproducibility simplifies technology transfer from research to commercial manufacturing and reduces the need to re‑optimize conjugation, blocking, or strip‑coating parameters when changing lots or scaling up production.

Storage and Expiration

• Citrate‑coated: store at 4–25 °C, protected from light. Do not freeze. 
• PEG‑COOH-coated: store at 2–8 °C, protected from light. Do not freeze. 

Products can typically be used for at least one year under recommended storage conditions. For extended storage, measuring UV‑Vis spectra is recommended to confirm colloidal and optical stability. 

Safety and Handling

NanoBrand does not guarantee the suitability of its products for any particular application. It is the customer’s responsibility to ensure that the product is appropriate and permitted for the intended use, in accordance with applicable regulations and internal quality systems. Please consult our Terms of Service and follow institutional guidelines for the safe handling and disposal of nanomaterials.

Discover Gold Nanoparticles - Spherical, Citrate Surface and Gold Nanostars.